| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | AaD | Club/Country | Time |
| 1 | 1 | Charlotte Tilley | 13 | Aquabears | 6:13.93 |
| 2 | 3 | Robyn Jacobs | 13 | Co Manch Aq | 5:49.50 |
| 3 | 5 | Lauren Airey | 13 | Preston | 5:34.40 |
| 4 | 7 | Lucy Turner | 13 | Burnley BOBC | 5:24.90 |
| 5 | 6 | Lydia Dewhurst | 14 | Burnley BOBC | 5:31.80 |
| 6 | 4 | Abbie Brennand | 15 | Bolton Metro | 5:38.50 |
| 7 | 2 | Mia Wroe | 12 | Burnley BOBC | 6:10.00 |